Journal: Tissue Engineering. Part C, Methods
Article Title: Microfluidic Encapsulation Supports Stem Cell Viability, Proliferation, and Neuronal Differentiation
doi: 10.1089/ten.tec.2017.0368
Figure Lengend Snippet: (A) Schematic representation of cell encapsulation within alginate-collagen microcapsules. The customized microfluidic device included one middle inlet for the introduction of the polymer solution containing the cell suspension. Microcapsules were produced by the shear force generated by the continuous phase formed by a laminar flow composed of MO and AA in mineral oil. (B) MO phase ( red ) acted as a shielding phase to prevent quick alginate gelation at the junction. Scale bar = 800 μm. (C) Highly monodisperse cell-laden alginate-collagen microcapsules of 440 ± 3 μm were produced with the customized microfluidic device. (D) Encapsulated NSCs growing in the form of neurospheres inside the alginate-collagen microcapsules ( white arrows ). (E) DPSCs were maintained as single cells within the microcapsules. Scale bars = 100 μm. AA, acetic acid; DPSC, dental pulp stem cell; MO, mineral oil; NSC, neural stem cell.
Article Snippet: Cells were encapsulated within alginate-collagen microcapsules of 440 ± 3 μm diameter with a customized microfluidic device coupled with controllable syringe pumps (KD Scientific, UK) , for fluid delivery.
Techniques: Produced, Generated